Lab 5 SP UST Webmail https Webmail of the beta sheet Explori

Lab 5 (SP. UST Webmail https Webmail of the beta sheet. Exploring the Model The i Can you trace the polymer chain from the data amino to carboxy terminus? Hint: the order PDB of the strands from amino to carboxy M. C terminal are marked with the numbers 1-11 R. Y in the arrowheads of each strand Cryst 2. What is the relationship between the strand green Scien labels A-K and 1-11? Comment also on the loops between the strands and location of the chromophore. 3. Identify one example each of parallel and antiparallel strands in the GFP barrel. Model of Green Fluoresce

Solution

Answer:

1. The amino terminal starts at NH₃+ with sequence of the beta strands being 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 and ends at COO- at the carboxy terminal. The beta strands will lie anti parallel to each other.

So, the primary sequence stands as:

NH3⁺- 1 - 2 - 3 - 4 - 5 - 6 - 7 - 8 - 9 - 10 - 11 - COO^-

2. Stand numbers 1-11 is the sequence of the beta strands according to the primary structure (amino acid sequence) of the protein.

Strands A-K represents the sequence of the beta strands in accordance with their relative orientation (arrangement with respect to each other) within the GFP molecule (model).

3. Strands K (1) and A (6) are parallel to each other in the model.

Strands K (1) and J (2) are antiparallel to each other.