Please explain your answer Thanks In order to determine the

Please explain your answer. Thanks!

In order to determine the overall chromosome map for a new wild type strain of E. coli, three Hfr strains were obtained by independent insertions of the F factor. The start times of transfer of several genes were determined during conjugation matings between each Hfr strain and an F recipient carrying six auxotrophic mutations. Using the times taken to begin transfer of each marker, draw a unified chromosome map of the chromosome of this strain of E. coli, showing its total length in minutes of transfer and the order and relative distances between these markers. Show the position and orientation of the F factor in each Hfr strain.

Solution

A. Hfr 1:

Ade--Bio----His--Met-----Ile

Hfr 2:

Met-His-----Bio-Ade

Hfr 3:

Bio-Ade-----------Lys--Ile

B.

Hfr 1: F factor-Ade------->

Hfr 2: F factor-Met------->

Hfr 3: F-factor-Bio------->