Which of the following statements are consistent with this g
Which of the following statements are consistent with this genotype?
MATa ura3 ade2 TRP2
This strain is mutant at the ADE2 locus
This strain is wildtype at the HOM3 locus
| This is a diploid strain |
Solution
Please fallow the statements and TRUE of FALSE against eact statement along with their reason:
1. The starin is diploid: FALSE
reason is:- if the strain will be diploid, two copies of the gene must belisted which are mating. Like MATa/MATa. But here only one copy of the gene is listed, so the strain is haploid.
2. The strain is ura+:- FALSE
the strain is negative for ura gene or we can say it is negative. because the positive genotype will be written as URA.
3. The strain is mutant at ADE2 locus: TRUE
This is a style of writing genotypes in the yeast, when different phenotypes are studied through complementation assay,so if it would be wild type, it should be denoted as ADE. if it is ade, it is a mutant genotype.
4. This strain can synthesize Tryptophan:- TRUE
Because mutant would be trp.
5. This is a wild strain for HOM3 locus.
