Homework SourseConsider the mRNA sequence 5 AAUGCAGCUUUAGCA 3 The sequence
Consider the mRNA sequence: (5\') AAUGCAGCUUUAGCA (3\'). The sequence of the coding strand of DNA is:
(5\') ACGATTTCGACGTAA (3\')
(3\') TTACGTCGAAATCGA (5\')
(5\') AATGCAGCTTTAGCA (3\')
(5\') AAUGCAGCUUUAGCA (3\')
(3\') AATGCAGCTTTAGCA (5\')
The 70 subunit of the E. coli RNA polymerase:
Acts as a catalytic site for polymerization.
Recognizes promoters.
Has a proofreading function.
Increases the processivity of the enzyme.
Recognizes termination signals.
| a. | (5\') ACGATTTCGACGTAA (3\') | |
| b. | (3\') TTACGTCGAAATCGA (5\') | |
| c. | (5\') AATGCAGCTTTAGCA (3\') | |
| d. | (5\') AAUGCAGCUUUAGCA (3\') | |
| e. | (3\') AATGCAGCTTTAGCA (5\') |
Solution
Anser 1.
Tho coding strand of the mRNA is the one strand of DNA which has exactly same base pairing with the newly synthesized mRNA molecule. ;
Therefore among the given options, the coding strand will be:
(5\') AATGCAGCTTTAGCA (3\')
This has same base pairing of the newly synthesized mRNA , only with the difference that T in DNA is replaced by U in mRNA.
Answer 2)
b. Recognises promoter
70 subunit of the E. coli RNA polymerase is housekeeeping region of promoter and it has proomoter recogniion sequence. and thus it helps in the efficient binding of polymerase on the promoter.
