Below is a diagram of DNA replication as currently believed

Below is a diagram of DNA replication as currently believed to occur in E. coli. From specific points, arrows are provided that lead to numbers. Answer the questions below relating to the locations specified by the numbers.

(1) Which end (5\' or 3\') of the molecule is here?

(2) Which enzyme is probably functioning here to deal with supercoils in the DNA?

(3) Which enzyme is probably functioning here to unwind the DNA?

(4) Which nucleic acid is probably depicted here?

(5) What are these short DNA fragments usually called?

(6) Which enzyme probably functions here to couple these two newly synthesized fragments of DNA?

(7) Is this strand the leading or lagging strand?

(8) Which end (5\' or 3\') of the molecule is here?

l. (5\' or 3\' end?) 2. (which enzyme deals with supercoils?) 7. (leading or lagging?) 3. (which enzyme?) 6. (which enzyme joins the nick?) 8. (which end 5\' or 3\'?)

Solution

1) 5 end

2) topoisomerase

3) helicase unwinds the DNA.

5) these are called as okazaki segment

6) ligase enzymes is used for joining.

7) lagging strand.

8) 3 end as the direction is 3-5.