cell nuclei experiment 1 What is function of SDS and O5M NaC

cell nuclei experiment

1. What is function of SDS and O.5M NaCl in the isolation of DNA

2. How did DNase affect the spooling of DNA?

3..What % of ethanol was used in the DNA isolation and where did stand when added to aqueous solution and why? it was 95% ethanol

Answer

1]In DNA isolation SDS is used for cell lysis & release of cell contents

Role of 0.5 M Nacl= The proteins & other hydrocarbons are still soluble in water but DNA form complex with Nacl salt at this conc.

2] DNAase affect the spooling of DNA =

Dioxyribonuclease breaks the phosphodiester bonds that connects the nucleotide units in DNA

degrades long DNA molecules into shorter segments

3] 95% ethanol is used in DNA extraction