Given the following DNA nontemplate coding strand determine

Given the following DNA non-template coding strand, determine the template strand sequence, mRNA sequence and the primary amino acid sequence coded. Write both the three letter and one letter amino acid code. 5\'- ATG GCT TCT ACC GAA AGA TAG Template strand Rewritten 5\'rightarrow 3\' mRNA Protein 3 letter Protein 1 letter

Solution

Non-coding strand= 5\'-ATGGCTTCTACCGAAAGATAG-3\'

Template strand= 3\'-TACCGAAGATGGCTTTCTATC-5\'

mRNA= 5\'-AUGGCUUCUACCGAAAGAUAG-3\'

Protein three letter - 3\'- UACCGAAGAUGGCUUUCAAUC- 5\'

UAC- Tyr, CGA- Arg, AGA-Arg, UGG-Trg, CUU- Leu, UCA-Ser, AUC- Ile

Protein one letter- Y R R W L S I