Primer design Given below is a single stranded DNA sequence

Primer design:
Given below is a single stranded DNA sequence. Design suitable reverse and forward primers that can be used to amplify the region highlighted here.

GTTCCATCAAGCAGACAGGTTTTGTGTTCGCGGGAACCACTATATTCACAACCTCTGATTGGAGTCGAGAAAACAAGAGGGGGCATATGCCTTTTAGAAAATACCTCAGCTCTGAATTCGGAGTTGCTGATACTCAAATAAACAGAAAAACAACTTTAGATAATCCGGCAATTTTGGAACAGCAATTAGAAGCTCTTGCTTACCATAAACTTCAAATGGAAAAAAAGGGTCTTTTGGGAGTACAAGCTAAGCCAACACAACCTCTCAACAGCTTTACAAAGCCCCTTTCAAAGACTTTAAGCAAAAGCTTAATATATTCTAATTTAGGTTCTGTTCGCAAAGAAATAGAAACATTAGAACTATTAACAGACGAAACTAAGATTCTGCGAGTACATATTCAAACGTTAATGAAACTG

GTTCCATCAAGCAGACAGGTTTTGTGTTCGCGGGAACCACTATATTCACAACCTCTGATTGGAGTCG AGAAAACAAGAGGGGGCATAT GAGTTGCTGATACT GCCETTTTAGAAAATACOTCAGCTCTGAATTCG CAAATAAACAGAAAAAACA ACTTTAGATAATCCGGCAATTTTGGAACAGCAATTAGAAGCTCTTGCTTA CCAT AAACTTCAAAT GGAAAAAAAAGGG TCTTTTGGGAGTACAAGCTAAGCCAACACAACCTCTCAACA GCTTTACAAAGCC(CCTTTCAAAAA GACTTTAAGCAAAAAG CTTAATATATTCTAATTTAGGTTCTGTTCGCA AAGAAATAGAAAACA TGCGAGTACATATTCAAACGTTAA TGAAACTG a. Keep in mind to select primers that are appropriate in length and GC content. Enter here the sequences of the primers you designed. (4 points) Forward primer Reverse primer

Solution

GCCTTTTAGAAAATACCTCAGCTCTGAATTCGGAGTTGCTGATACTCAAATAAACAGAAAAACAACTTTAGATAATCCGGCAATTTGGAACAGCAATTAGAAGCTCTTGCTTACCATAAACTTCAAATGGTCTTTTGGGAGTACAAGCTAAGCCAACACAACCTCTCAACAGCTTTACAAAGCCCCTTTCAAAGACTTTAAGCAAAAGCTTAATATATTCTAATTTAGGTTCTGTTCGCAAAGAAATAGAAACA

Reverse primer -

Note- The highlighted regions in the template to be amplified, show the position where the forward and reverse primers will attach. The primers are designed according to basic primer designing rules which follow the specific condition that GC content in primers should be between 40-60% and primers should not attach anywhere else in the sequence.