To answer this quesiton you will have to research these enzy

To answer this quesiton, you will have to research these enzymes on the NEB site. Drawings will be essential to help you answer this question, and will prepare you better for the exam.

You cut the vector with XmaI and EcorI.

You cut the PCR product with AgeI and EcorI.

Now you ligae the cut PCR product with the cut vector. What happens?

A)The ligation succeeds because even though XmaI and AgeI recognize different sites, the sticky ends they generate are compatible.

B)The ligation succeeds because even though XmaI and AgeI are different enzymes, they recognize the very same restriction site.

C)The ligation succeeds because, as evident from NEB site analyses, the EcorI site from the PCR will match up with AgeI site from the Vector, and the EcorI site from the Vector will match up with the XmaI site on the PCR.

D)The ligation fails because XmaI and AgeI are different restriction enzymes.

The ligation succeeds because, as evident from NEB site analyses, the EcorI site from the vector will match up with AgeI site from the PCR, and the EcorI site from the PCR will match up with the XmaI site on the vector.

Solution

The answer is D)The ligation fails because XmaI and AgeI are different restriction enzymes.as the following are the sites and the complementary ends they generate in bold. Any of the combinations above will not lead to sticky ness in the DNA and the ends are not compatible.

Xmal

CCCGGG GGGCCC

ECORI

GAATTC CTTAAG

AGEI

ACCGGT TGGCCA

ECORI

GAATTC CTTAAG