Mitochrondrial enzyme and cytochrome C oxidase activity lab

Mitochrondrial enzyme and cytochrome C oxidase activity lab

1. which components of cytochromec oxidase transfer the electrons to oxygen?

2. what is the purpose of using a positive control for the assay?

Solution

1.Cytochrome C oxidase catalyzes the reduction of molecular oxygen to two molecules of water a process which requires transfer of four electrons to oxygen. Four Electrons going to oxygen come from cytochrome c.in cytochromec oxidase

2.The cytochrome c oxidase assay kit allows for detection of intact mitochondria by assessing the outer membrane integrity via cytochrome c. Pure mitochondrial fractions containing intact mitochondria will yield a positive change in absorption. A positive control is thus used to show large amount of enzyme activity.