12 You want to extract dsDNA from live tissue Which of the f

12. You want to extract dsDNA from live tissue. Which of the following is an option, not a necessity?

A. SDS

B. RNase A

C. S1 nuclease

D. Exonucleases

Solution

Option B. RNase A. It is used to denature RNA and extract DNA in pure form.

S1 nuclease is used to extract single stranded DNA or RNA.

SDS is absolutely necessary for DNA extraction, so it is not an option but a necessity.