You are recording from a pair of neurons You suspect that th

You are recording from a pair of neurons. You suspect that they are connected by an electrical synapse. Describe four experiments that you could use to test whether the connection is electrical rather than chemical. If you remove calcium in the extracellular medium, what will happen at this synapse in terms of impulse propagation? How do you calculate the coupling coefficient of two cells connected by a gap junction and what does this tell you quantitatively?

Solution

The name of 4 experiments that could be used to test the connection of neurons is electrial rather than chemical are-

1.voltage regulator byalan hodkin, 2.patch clamp technique byneher and sakmann, 3.adrian experiment, 4.eric candel experiment.

B.Calcium promotes vesile fusion with terminal membrane and release of transmitter acetylcholine it diffuse through the cleft and changes the permeability and membrane potential in post synapti membrane. so this funtion will stop in ca absence .

c. COUPLING COEFFICIENT= JUNCTION CONDUCTANCE UPON( JUNCTION CONDUCTANCE+MEMBRANE CONDUCTANCEC OF UNINJECTED CELL.

By this one way transmission have been observed in both vertebrate and invertebrate.