what is MLC how is it different from MIC can the results of

what is MLC? how is it different from MIC? can the results of a MIC test be used to determine whether an antibiotic is bactericidal or bacteriostatic? and what can be found in the zone of inhibition?

Solution

what is MLC? how is it different from MIC?

Minimum Inhibitory concentration test (MIC): This test is used to measure the lowest effective concentration of antibiotic against the growth inhibition of some specific bacteria.

MBC or MLC: minimum bactericidal concentration is referred as the lowest concentration that is required to microbial death of 99.9% of the original inoculum

Yes, the results of a MIC test be used to determine whether an antibiotic is bactericidal or bacteriostatic, the antibiotic is going to enter through the process of “diffusion” into the agar from the “disk immersed in antibiotic” placed into the agar form the region of high concentration to region of low concentration. At the time of incubation, the antibiotic is going to emerge into agar in which edge of zone of inhibition depicts MIC, but not the end of diffusion.

Kirby-Bauer disk susceptibility test to test effectiveness of bactericidal or bacteriostatic agent: In this antibiotic is placed in disks in nutrient media and tested for the growth of specific antibiotics. The microbe free zones surrounding the wells with antibiotic are taken as criteria to measure the effectiveness.

what can be found in the zone of inhibition?

Zone of inhibition test: In a nutrient agar media containing \"microbial growth\", the zone of inhibition is tested with the test antibiotic drug. At the time of incubation, the antibiotic is going to emerge into agar in which edge of zone of inhibition depicts \"MIC\", but not the end of diffusion

In this case, zone of inhibition’s edge is not considered as limit of antibacterial agent diffusion into the agar medium. This is because diffusion of anti-microbial agent occurs beyond the edge based on the concentration of “antibiotic used”. However, the edge represents meticulously “minimum inhibitory concentration” of antibacterial agent (for example, bacitracin)