Answer the following questions A single strand of bacterial

Answer the following questions: A single strand of bacterial DNA contains the base sequence 5\' TGTATTCATGAGCCACTATCGTATATTCCCTGCTTGAGTATCATTATTCCTCCT 3\' Write the complementary sequence below the given sequence with its polarity (5\'-3\' ends). Write the 6 reading frames below (group nucleotides in codon, i.e. ACGCVGTG ... = ACG CVG TG. ...) ? Is there an open reading frame (ORF) at least 4 codons long? If yes, what is the reading frame (+1, +2, +3, -1, -2 or -3) for the ORF? Does the strand of the DNA given in (a) serve as the coding strand or template strand for the synthesis of the RNA transcript ?

Solution

A.

5\'TGTATTCATGAGCCACTATCGTATATTCCCTGCTTGAGTATCATTATTCCTCCT3\'
3\'ACATAAGTACTCGGTGATAGCATATAAGGGACGAACTCATAGTAATAAGGAGGA5\'

B.

5\'TGT ATT CAT GAG CCA CTA TCG TAT ATT CCC TGC TTG AGT ATC ATT ATT CCT CCT3\'

5\'T GTA TTC ATG AGC CAC TAT CGT ATA TTC CCT GCT TGA GTA TCA TTA TTC CTC CT3\'

5\'TG TAT TCA TGA GCC ACT ATC GTA TAT TCC CTG CTT GAG TAT CAT TAT TCC TCC T3\'

3\'ACA TAA GTA CTC GGT GAT AGC ATA TAA GGG ACG AAC TCA TAG TAA TAA GGA GGA5\'

3\'A CAT AAG TAC TCG GTG ATA GCA TAT AAG GGA CGA ACT CAT AGT AAT AAG GAG GA5\'

3\'AC ATA AGT ACT CGG TGA TAG CAT ATA AGG GAC GAA CTC ATA GTA ATA AGG AGG A5\'

C.

>123: 2 to 34: Frame 2 11 aa
VFMSHYRIFPA
>123: 54 to 19: Frame -1 12 aa
RRNNDTQAGNIR
>123: 53 to 9: Frame -2 15 aa
GGIMILKQGIYDSGS
>123: 35 to 49: Frame 2 5 aa
VSLFL
>123: 12 to 53: Frame 3 14 aa
ATIVYSLLEYHYSS
>123: 43 to 5: Frame -3 13 aa
YSSREYTIVAHEY
>123: 1 to 54: Frame 1 18 aa
CIHEPLSYIPCLSIIIPP
>123: 15 to 1: Frame -1 5 aa
WLMNT

d. Yes