We will now look for the OREs of a given sequence CGCTACGTCT

We will now look for the OREs of a given sequence. CGCTACGTCTTACGCTGGAGCTCTCATGGATCGGTTCGGTAGGGCTCGATCACATCGCTAGCCAT How many reading frames does this sequence contain? Write down each of the frames with the equivalent sequence. How many ORF are there? Indicate where they are.

Solution

There are 21 reading frames .They are:

1.CGC

2.TAC

3.GTC

4.TTA

5.CGC

6.TGG

7.AGC

8.TCT

9.CAT

10.GGA

11.TCG

12.GTT

13.CGG

14.TAG

15.GGC

16.TCG

17.ATC

18.ACA

19.TCG

20.CTA

21.GCC

Open reading frames are frames that can be translated.They are:

1.TGG

2.GGA

3.TAG

4.GCC