1 How do enzyme inhibitors help us investigate how enzymes i

1. How do enzyme inhibitors help us investigate how enzymes interact with their endogenous substrates?

2.Why are MAO inhibitors useful for treatment of Parkinson’s Disease? It may be necessary to research the physiology of Parkinson’s Disease to answer this question.

Solution

1)The binding of an inhibitor can stop a substrate from entering the enzyme\'s active site and or hinder the enzyme from catalyzing its reaction. Inhibitor binding is either reversible or irreversible. Irreversible inhibitors usually react with the enzyme and change it chemically. These inhibitors modify key amino acid residues needed for enzymatic activity. In contrast, reversible inhibitors bind non-covalently and different types of inhibition are produced depending on whether these inhibitors bind to the enzyme, the enzymes endogenous substrates.

In this inhibition assesment methods can be used.....

2)Monoamine oxidase inhibitors belong to the earliest drugs tried in Parkinson\'s disease reversible MAO-A inhibitors are recommended due to their easier clinical handling.Loss of dopamine, noradrenaline and serotonin is the biochemical basis of degenerative processes underlying Parkison disease.Both, selegiline and rasagiline are beneficial in treating motor symptoms in PD as monotherapy and in combination with L-DOPA and a decarboxylase inhibitor.