2 In a generalized transduction experiment using P1 phage th

2. In a generalized transduction experiment using P1 phage, the donor is pur+nad+ pdx- and the recipient is pur- nad- pdx+. After transduction, pur+ is selected, and 50 transductants are scored for other phenotypes present, with the following results: nad+ pdx+ 3

nad+ pdx- 10

nad- pdx+ 24

nad- pdx- 13 a. What are the cotransduction frequencies for each gene? b. Which of the unselected genes is closest to pur? c. Are nad and pdx on the same or opposite sides of pur?

Solution

Answer:

13 a. The cotransduction frequencies for pur and nad is 13/50=26%.

The cotransduction frequencies for pur and pdx is 23/50=46%.

b.pdx is closest to pur.

c. nad and pdx are on the same sides of pur.