Flasmid Restriction Map EcoRI BamHI BamHI pMCB3023 BamHI Hae

Flasmid Restriction Map EcoRI, BamHI. BamHI pMCB-3023 BamHI Haelll. Pstl estion 1 Pstl Sau3Al EcoRI. Scale Sau3 Al

Solution

ANSWER:

1)Identify the enzyme that will generate a linear molecule of double stranded DNA after
a full digestion of the plasmid pMCB­3023.

RESTRICTION ENZYME ECORI.......

2)How many fragments will be created after a double digestion with SmaI and EcoRI restriction ezymes?

THREE FRAGEMENTS.

3) After the double digestion was accomplished the DNA mixture was analyzed by gel electrophoresis. Which of the following pictures better describe the expected
bands in the agarose gel?

IT IS C .......................................