3 Write the calculations to make the following solutions usi

3. Write the calculations to make the following solutions using the stocks and solids listed above. Assume all the above solutions are in deionized water.

a. How would you prepare a 500 mL solution containing 1.5 M Tris and 20% KCl?

b. How would you prepare a 0.75 L solution containing 50 mM NaCl and 10% SDS?

c. How would you prepare a 100 mL solution containing 1% agarose and 1x TBE?

d. How would you prepare a 250 mL solution containing 250 mM Tris, 5 g/L NaCl, and 50% SDS?

E. How would you prepare a 200 L solution containing 1% glycerol?

A. You are given 50 µL of a highly concentrated DNA solution. You need to prepare 1.0 mL of a 1:500 dilutions. Describe how you would prepare this solution

B. You diluted a DNA solution 1:100 and measured the OD at 260 nm. The spectrophotometer gave you a reading of 0.35. Describe how would you determine the DNA concentration of your original solution? Remember, the _DNA = 50x10^-3 mg/mL.

Solution

we will apply the molarity formula

M=(Given mass * 1000)/ (molecular mass * volume of solutions(ml))

A) according to this formula,

M=1.5 M

Volume of solutions =500 ml

Molecular mass = as given..

We can calculate the given mass required to dissolve in this amount of water to obtain the 1.5M... To that if we 100 gm of KCl we will get the desired solution..!!

B) similarly we can also find the weight of the NaCl required to dissolve in 0.75L to obtain 50mM...!! To that add 75 gm of SDS..!!

C) with the above given formula, we can put the value in the formulae we can calculate the given mass required to dissolve in this amount of water... Add 1gm of agarose in it..