RNA has been isolated for gene expression analysis A total v

RNA has been isolated for gene expression analysis. A total volume of 10 µL RNA was measured at 1596.2 ng/µL. In order to complete the gene expression analysis, a final concentration of 200 ng/µL is necessary. How much water must be added to the RNA to reach this concentration?

Solution

Answer:

Initial concentration = 1596.2 ng/microL, Final concentration = 200 ng/microL

Using V1S1 = V2S2, we get

1596.2 * V2 = 10 microL * 200

Therefore, V2 = 2000/1596.2 = 1.293 microL

So, 1.293 microL of the sample should be added with 8.707 microL of water to get a concentration of 200 ng/microL.