How many subunits the protein W has Why proteins II III and

How many subunits the protein W has? Why proteins II, III and IV were eluted in three different fractions from the column when comparing their corresponding protein bands in SDS-PAGE A? Attach the log MW vs Rf graph and MW calculations for the unknown proteins A and B. The MWs of unknown proteins are calculated from their Rf values using an equation that you will get from a linear fit to the log MW vs Rf graph (calibration graph). The Rf values for the standard proteins separated on your gel are used to construct the calibration graph. The molecular weights of standard proteins are: 170, 135, 100, 72 (red band), 55, 40, 33, 24, 17, 11 kDa.

Solution

Since the molecular weight of protein W has common in both the figure, i.e. 117 kD

So, as per Part A, the protein W has 6 subunits and as per Part B, the protein W has 4 subunits.

The fractions of protein in SDS PAGE are depend upon the procedure of the electrophoresis. In Part A, it is obvious that the fraction of protein II, III and IV are seperated so easily while in Part B, it is difficult to seperate out the fractions of the protein.