Use the PopSet population study data sets in ENTREZ to retri

Use the PopSet (population study data sets) in ENTREZ to retrieve coding sequence

(CDS) of amylase-related gene (amyrel) generated for Drosophila yakuba by Cariou et al.

(2001). You should retrieve 5 Drosophila yakuba sequences from different population strains.

***Make sure all your sequences are Drosophila yakuba.***

(a) Report the GenBank accession numbers.

(b) Align coding regions of the 5 sequences. Which alignment software did you use?

(c) Look at the first 200 bases of the alignment. Count the numbers of segregating sites(K),

the numbers of segregating sites per sites (k), and nucleotide diversity ().

Solution

Entrez is a global query cross database search system or it is a web portal that allows users to search many discrete health sciences databases at NCBI ( National Center for Biotechnology Information).

Sequence 2:

1 atgttcaagt tggctttgac cctgacactc tgcttggcgg gcagcctctc gctggcccag

Sequence 3:

Sequence 4:

Sequence 5: