Jessica streaks out 4 different streak plates but doesnt get

Jessica streaks out 4 different streak plates, but doesn\'t get isolated colonies on any of them. She makes the same mistake on all of them. What is the most likely mistake Jessica continues to make?

She takes too much inoculum from the original culture for the first section.

She doesn\'t flame her loop between each section.

She reinoculates her loop from the original culture for each section.

She takes too little inoculum from the original culture for the first section

Solution

B.In the streaking procedure, a sterile loop or swab is used to obtain an uncontaminated microbial culture. The process is called \"picking colonies\" when it is done from an agar plate with isolated colonies and is transferred to a new agar or gelatin plate using a sterile loop or needle. The inoculating loop or needle is then streaked over an agar surface. On the initial region of the streak, many microorganisms are deposited resulting in confluent growth or the growth of culture over the entire surface of the streaked area. The loop is sterilized by heating the loop in the blue flame of the Bunsen burner, between streaking different sections, or zones and thus lesser microorganisms are deposited as the streaking progresses. The streaking process will dilutes out the sample that was placed in the initial region of the agar surface. T