Which methodology may be used for detection of incorporated

Which methodology may be used for detection of incorporated BrdU?

A. colorymetic or chemilumnescence ELISA B. fluoroscence reading in fluorescence plate reader or cell counting by flow cytometer C. counting of stained nuclei under fluorescence microscope D. all of the above

Solution

The above three methods can be used for incorporate BrdU detection.

BrdU ie bromodeoxyuridine is used for the detection of proliferating cells.BrdU can be incorporated in to replicating DNA as a substituent for thymidine and its presence can be detected using the above immunoassays