Why is agar called to 50C prior to being inoculated with bac

Solution

Agar is cooled to 50°C prior to being inoculated with bacteria because at too high temperature, the bacterial membrane will be disrupted and bacteria will die from cell lysis. At a too lower temperature, the bacteria fails to diffuse through agar media and it does not support the bacterial growth because the enzymes necessary for bacterial growth are not activated at lower temperature. The appropriate temperature for many of the bacteria typically ranges from 25 degrees centigrade to 50-degree centigrade. So, the agar media must cooled to 50°C prior to inoculation.