Answer the question below A 3T3L1 fibroblasts What are they

Answer the question below: A) 3T3-L1 fibroblasts: What are they? What must you do to get them to differentiate? What are signs that they have differentiated? (http://www.atcc.org/)

Solution

3T3-L1 fibroblasts: What are they?

3T3-L1 is a cell line derived from adipose tissue of mouse. These cell lines have enhanced secretion and accumulation of triglycerides.

What must you do to get them to differentiate?

Please note that the actual differentiation process takes several weeks to occur e.g. 2 - 5 weeks.

Signs of differentiation

In starting, patch formation occurs. This is the first sign of differentiation. As time proceeds, a significant percentage of the population of cells will change.