A DNA sample and a plasmid with the ampR and lacZ for the en

A DNA sample and a plasmid with the amp^R and lacZ (for the enzyme beta-galactosidase) genes are treated with Notl with the goal of making recombinant DNA molecules. The lacZ gene is placed in the cloning region so that it is disrupted by DNA inserts. Put the remaining steps for this process in the correct order. Not all statements will be used. All the fragments have identical sticky ends: 5\'-GGCC-3\'. DNA ligase joins the sticky ends of the DNA sample and the plasmid White colonies that survive on the culture medium are identified as having the recombinant plasmid Colonies containing the recombinant plasmid can be removed from the culture medium and grown Cells containing the recombinant plasmid are grown in a medium with ampicillin and X-gal (a galactose derivative) Blue colonies that survive on the culture medium contain the recombinant plasmid All the fragments have the sticky ends 5\'-GCGGCCGC-3\'.

Solution

1.) All the fragments have identical sticky ends. 5\' -GCGGCCGC-3\'

2.) DNA ligase joins the sticky end of the DNA sample and the plasmid.

3.) Cells containing the recombinant plasmid are grown in a medium with ampicillin and X-gal ( a galactose derivative).

4.) White colonies that survive on the culture medium are identified as having the recombinant DNA.

5.) Colonies containing the recombinant plasmid can be removed from the culture medium and grown.