Fibronectin collagen type 1 Gelatin polylysine bovine serum

Fibronectin:

collagen type 1:

Gelatin:

poly-lysine:

bovine serum albumin (BSA):

1. Predict which of the above substrates will support the highest rate of cell proliferation? Be sure to justify your hypothesis.

2. How much do the substrates listed above cost? Complete the table below for 24 wells of a 24-well culture plate. Substrate Cost Fibronectin Collagen Type 1 Gelatin poly-lysine BSA

Solution

Poly -L-Lysine and FN-coated wells increase the attachment of prostate cancer cell lines cells.

Out of these fibronectin shows increased attachment.

Increased attachment is responsible for increased proliferation.

Cost of products:

Fibronectin - 13,203 rupees

Collagen type 1 - 3117 rupees

Gelatin - 2684 rupees

poly- lysine- 9449 rupees

BSA - 4380 rupees