values in Exercise 4 record those values in the table and ab

values in Exercise 4, record those values in the table. and absorbance values for your unknown sample e last row. Finally, make sure you record the letter on your Absorbance Transmission 88+a 0.00075 100 page to illustrate the relationship between the amount of known concentrations of flavin in those tubes. (See Appendix the uses preparations of graphs.) In these experiments, and absorbance flavin and measured transmission as well as of transmission and absorbance, therefore, were dependent tly, transmission and absorbance are the dependent (unknown) h we chose, are the independent (known) variable values (they mental variables). As is customary for graphic presentations, variable to the y

Solution

For the ease of plotting the graph, the transmission values and absorbance values need to be corrected upto 2 decimal places. You had probably taken 2 readings per concentration and have found the average, which is fine.

So you need to plot seperately transmission vs concentration and absorbance v/s concentration.

You will have to plot these values in the given template graph.

After plotting all the points, from both the graph, you will have to find out the concentration of the Unknown. For this, extend a line touching the point of absorbance or transmission of the Unknown onto the best fit line and extrapolate that line on the X-axis.

Concentration of flavin (mM)	Transmission
0.12	2.50
0.06	90.85
0.03	39.15
0.015	58.45
0.0075	78.90
0.0	100
Unknown	79.0