Restriction endonucleases simply cut the backbone of DNA at

Restriction endonucleases simply cut the backbone of DNA at specific sequences. Why are these enzymes of such importance to molecular biologists and genetic engineers? (Hint: Explain how such enzymes might have been used to create the biological components we used in this lab.)

Solution

Restriction endonucleases work by cutting the DNA in a specific internal site. They create blunt or sharp ended- smaller DNA fragments. These are important for molecular biologists and genetic engineers because they help in creating new genetic combinations as well as studying the DNA of a person. For example, these enzymes help in making RFLP\'s, which help in predicting a unique pattern of a person\'s DNA and in his/her identification. We can use these results for comparison as well. For plasmid construction, these enzymes play a key role. This is how these scissors are very important in genetics.