Describe in words the role or characteristics of each of the

Describe in words the role or characteristics of each of the following in prokaryotic transcription:

a) promoter region

d) RNA polymerase holoenzyme

b) –10 region

e) sigma () factor

c) –35 region

f) ribonucleoside triphosphates (NTPs)

Next, try to construct a narrative of prokaryotic transcription, using each of the terms above as appropriate. Make a list of all additional technical terms you use in your narrative.

Solution

Promoters in prokaryotic organisms are equal to their eukaryotic TATA box. They are two short DNA sequences located at the -10 (10bp 5\' or upstream) and -35 positions from the transcription start site (TSS).

RNA Polymerase, Holoenzyme initiates RNA synthesis from sigma 70 which is the core enzyme saturated with sigma factor 70.

In bacteria, the promoter contains two short sequence elements one of that is approximately -10 (Pribnow Box) which has the consensus sequence TATAAT.

the another sequence is the sequence at -35 (the -35 element) has the consensus sequence TTGACA.above promoter sequences are recognised through RNA polymerase holoenzyme containing sigma-70. and initiate the synthesis of RNA

ribonucleoside triphosphates (NTPs), they arerequired during synthesis of RNA or DNA.