TrueFalse RNA polymerase copies both strands of DNA during t

True/False _____RNA polymerase copies both strands of DNA during transcription. _____RNA polymerase starts transcription at the Start Codon and stops at the Stop Codon. _____Bacterial RNA polymerase requires sigma-factor to effectively initiate transcription. _____RNA polymerase generates Okazaki Fragments on the Lagging strand. _____RNA polymerase copies the entire genome before every cell division. RNA polymerase initiates transcription of the double stranded DNA given below at the indicated transcription start site. Write the sequence of the resulting RNA in the 5\' to 3\' direction into the box given below. You perform 4 DNAsel footprinting experiments. All the experiments contain DNA 1. Experiments 2, 3, and 4 also contain Protein A which binds DNA 1 in the indicated position. DNA 1 is labeled by radio labels on the indicated ends. Arrows show positions that DNAsel can cut. Draw the banding pattern you expect to see in the 4 different experiments in the box on the right.

Solution

Answer :

4. Transcription

   1. This statement is FALSE, since the RNA polymerase Reads only one of the strands of the DNA called template strand.

2. This statement is FALSe, since the RNA polymerase strats transcription at the site of promoters and ends at the termination sequences.

3. This statement is TRUE.

4. The given statement is FALSE, since RNA polymerase is nothing to do with the Okazaki fragments. It is the DNA polymerase that generated okazaki fragments in the lagging strand synthesis during DNA replication.

5. The given statement is FALSE. Since the DNA polymerase not the RNA polymerase that copies the entire genome before the cell division.