Bio cell and molecular answer ALL 1 Which of the following i

Bio: cell and molecular

answer ALL

1 Which of the following is NOT true about hydrophobic interaction chromatography?

A the solvent is hydrophobic

B the column is hydrophobic

C proteins that are hydrophobic tend to be retained in the column

2 can you predict what would happen if any colony from the LB/amp/ara plate were streaked on an LB/amp plate?

A the colonies would not grow because there is no ara

B the colonies would change from the non fluorescent to fluorescent

C the colonies would change from fluorescent to non fluorescent

3 in the second centrifugation ( after cell lysis), the pellet is expected to be predominantly_________

A bacterial cell wall, membrane, nuclei

B bacterial cells

C pGLO plasmid

4 look over the experimental protocol. Why do you discard the supernatant from the first centrifugation?

A the pellet contains untransformed bacteria

B the supernatant contains bacterial debris from lysis

C the pellet contains the bacteria with GFP within

5 explain the purpose of using a dry ice bath in the GFP purification protocol?

A cooling the cell prevents breakdown of the desired GFP

B cooling the cell results in expansion of the cytoplasm

C cooling the cell results in shrinkage of the cytoplasm

1. A.

Correct answer is: Solvent is hydrophilic. High salt solutions are used for HIC in a gradient manner.

2. C

Arabinose is a sugar that turns on the gene for fluorescent protein. In the absence of arabinose in media, all colonies will turn to non-fluorescent.

3. A

Cell lysis breakdown cell membrane. Thus, centrifugation will septate cell debris, cell membrane and cell wall in the form of pellet.

4. C.

5. C.