True ORF Clone pCMV6ANMyc GIPtagged 59 kb shown above are tw

True ORF Clone pCMV6-AN-Myc. GIP-tagged 5.9 kb. shown above are two typical vectors that are used tpr producing proteins in mammalian cells. a. Please identify features of these molecules th ape unique to mammalian vectors vs those that are used only in E. coli. Explain each very briefly Only mammalian vector haue promoter cuv) b. Which of them would be bette or determining the cellular tion of the protein that is being produced in the mammalian ne? Please explain briefly.

Solution

okay let me explain it to u

mammalian vectors have

kozak sequences and sv40 origin of replication..... as mammals are eukaryotic organisms and kozak sequence is only found in eukaryotic transcripts ..... where as prokaryotic have TATA box consensus promoters. Similarly simian virus 40 (sv40) promoter wors only in mammals... as derived from simian virus which infects humans (mammals)

where as in ecoli

f1 origin of replication from phage infecting ecoli

similarly COLE1 is form bacterial plasmids andstands for colicin E1 gene .....

Hope it answers your first part

comming to second part

gfp stands for green flourescent protien ... it glows(shows flourescence) when exposed to light in the blue to ultraviolet rang and is attached to protien sequence as shown above, so where ever there is protien in cell that part glows up and that glowing region shows us the localisation of protien within cellular organeles ....where as myc tag doesnt glow so cannot show localisation ..... so vector with gfp can be used for localisation/location studies .....hope it helps