List four FeS scaffold proteins that can transfer ironsulfur

List four (Fe-S scaffold) proteins that can transfer iron-sulfur clusters to apoproteins and convert apoproteins to holoproteins in the plastids (e.g., chloroplasts). (Reference: Couturier et al. [2013] Frontiers in Plant Science 4: 259) Describe the experimental procedure to determine the subcellular localization of NFU-green fluorescent protein (GFP) fusion proteins. (Reference: Leon et al. [2003] Biochemical Journal 371: 823-830) Use your knowledgement of importance of Fe-S clusters in embryo development and seed setting, explain why an AtHscB T-DNA insertion mutant shows reduced seed set. (Reference: Xu et al. [2009] PLoS ONE 4: e7662)

Solution

The four protein are followings:-