One component of the Minimum Inhibitory Concentration experi

One component of the Minimum Inhibitory Concentration experiment is the creation of an antibiotic serial dilution. In this assignment, you are to determine the appropriate dilution from the original stock needed to reach the final concentration of antibiotic in 1 mL of media. In your experiment, you are to add only 10 mu L of antibiotic to the media. You are given the starting concentration of the antibiotic (the stock solution) and the final concentrations needed. The first three answers are given.

Solution

the dilutions will be as follows:

1:2.5, 1:2, 1:1.5, 1:1, 1:0.5, 1:0.25

Therefore, the final dilution of 1:0.25 will correspond to the final concentration of 0.02 µg/µL of diluted antibiotic.