Please show your work and explain your steps Thank you 8 15

8. (15 points) In this problem we will considerastudy on insertion of common repeating seq in ollagen. The most looked at the insertion of DDG collagen is Pro-Hyp-Gly. A paper in 2013 published in Biomolecules terminus in collagen peptides. These insertions were added at the N- and C-terminal regions of the peptide as follows: N-terminus addition: (Asp-Asp-Gly 3-(Pro-Hyp Gly)7 C-terminus addition: (Pro-Hyp-Glyon-(Asp-Asp-Gly)3 (a) Describe the structure ofnormal collagen. b) LOOKAT THE DATA ATOM Naci. Are these mutants more or less stable than the original? Briefly explain, giving a plausible explanation for your answer. The Tm data is as follows: 0 M Naci 100 mM NaCl 500 mM NaCl 38 deg. C NIA NA Normal collagen terminalinsertion 175 deg C 24.8deg. C 29.4 deg C Cterminalinsertion 208 degC 26.2 deg C 318 C (c0 What happens as the ionic strength is increased? Explain why this happens!

Solution

1.

b.

3. The number of moles can be calculated from handerson hasselbatch equation from the formula

pH = pKa + log [ A^-]/ [HA]

pH = pKa + log [ HPO4] [Na]² / [Na2HPO4] ------------------------ Exists as conjugate base

pH = pKa + log [ H3PO4] [Na]² / [Na2HPO4] -------------------------- exists as conjugate acid

Upon substituting the above given values gives the value of conjugate acid and base.