You mistakenly confuse the primary stain and counterstain Yo

You mistakenly confuse the primary stain and counterstain. You initially stain the smear with safranin, add iodine, and then decolorize and counterstain with crystal violet. How does your mixed culture now appear when viewed with oil immersion? ______

Solution

5. All the cells will appear purple at the end of the procedure. That is all cells will appear as Gram positive cells.

This is because, in the decolorization step, the alcohol will drain out all safranin since mordant will not work with safranin, and as crystal violet will be used as counter stain all cells will take up crystal violet and turn purple.