Mutation of Tyr55 to Phe decreases kcat only 43 fold while m

Mutation of Tyr-55 to Phe decreases k_cat only 4.3 fold, while mutation of Tyr-14 to Phe resulted in a 10^4.7 fold decrease and mutation Asp-38 to Asn resulted in a 10^5.7 fold decrease in rate of catalysis.

Which amino acids are most important to the mechanism?

Solution

Answer:

Aspartate is the most important for the mechanism because the mutation Asp-38 to Asn causes highest decrease in rate of catalysis.

kcat describes the turnover rate of an enzyme-substrate complex to product and enzyme.