a transcribe and translate this gene b translate the coding

a. transcribe and translate this gene

b. translate the coding region of the gene shifting the frame +1 and +2. (do this for the first 10 codons or until you encounter a stop codon).

Solution

translation of the sequence gives the following protein sequence--ATG CAC CGG ATC ATG CGG GAT CTC ACC AGC CTG GGG AGG GAG GGA GGT CTC CTG AAG ATC

a)5\'3\' Frame 1
Met H R I Met R D L T S L G R E G G L L K I

5\'3\' Frame 2
C T G S C G I S P A W G G R E V S Stop R

5\'3\' Frame 3
A P D H A G S H Q P G E G G R S P E D

3\'5\' Frame 1
D L Q E T S L P P Q A G E I P H D P V H

3\'5\' Frame 2
I F R R P P S L P R L V R S R Met I R C

3\'5\' Frame 3
S S G D L P P S P G W Stop D P A Stop S G A

Original sequence:

> - 60 nucleotides
atgcaccggatcatgcgggatctcaccagcctggggagggagggaggtctcctgaagatc

Translation in forward direction:

frame +1
> - 20 codons
MetHisArgIleMetArgAspLeuThrSerLeuGlyArgGluGlyGlyLeuLeuLysIle

Sequences alignment:

> - 60 nucleotides
Translation in forward direction:

DNA:  atgcaccggatcatgcgggatctcaccagcctggggagggagggaggtctcctgaagatc
+1fr: MetHisArgIleMetArgAspLeuThrSerLeuGlyArgGluGlyGlyLeuLeuLysIle


DNA:  
+1fr:

frameshift site--http://genoweb.toulouse.inra.fr/FrameD/FD2

  1  

  UACGUGGCCU AGUACGCCCU AGAGUGGUCG GACCCCUCCC UCCCUCCAGA GGACUUCUAG