An undergraduate biochemistry student was working on glutath

An undergraduate biochemistry student was working on glutathione enzyme from rat liver after exposing the experimental animals to toxic levels of cadmium for use the effect of cadmium toxicity on antioxidant status in the rat. She obtained the following results during extraction and characterization of the enzyme. Calculate the total activity, total protein. concentration, specific activity, percent yield and purification fold. Indicate their units of measurements. Fill all the blank spaces.

Solution

Enzyme activity= amount of product formed per unit time, which has SI unit =

1 katal= 1 mol/sec, 1 nkaltal = 1nmol/sec

Specific activity = activity/protein concentration

Total activity = activity x Volume

Total protein concentration = Volume x protein concentration

Yield = Activity / Total activity                     [x 100 for yield percentage]

For 1^st filtration the fold purification would be 1 and then for next purifications use the following formula:

Specific activity/ 1^st filtration specific activity.