Plasmid pRIT452 was cut with Pstl HindlII and EcoRI From the

Plasmid pRIT452 was cut with Pstl. HindlII, and EcoRI. From the data below, determine the map Pstl HindIll. 6.2 EcoRI PstI HindIII 4.8 2.0 1.7 Pstl EcoRI 5.4 3.8 3.0 EcoRI HindlII 6.2 1.8 1.2 Plasmid pRIT453 was cut with smal, Hindlu, and EcoRI. From the data low, determine the map. EcoRI 1.6 Hindlll 1.9 Smal 6.6 EcoRI Hindlll 4.5 1.9 1.6 EcoRI Smal 5.7 0.7 Smal HindIII 2.7 2.2 1.9 e the ry on only des o nega

Solution

figure shows pRIT452 map, in the same way ,it can map pRIT453 also. first draw a gel running like picture then compare fragment with different enzyme and find out exact position accordingly