Model 2s DNA 5A G A G G T G C T 3 POSTACTIVITY skLL EXERCISE

Model 2\'s DNA

5\'-A G A G G T G C T -3\'

POSTACTIVITY skLL EXERCISES 1 Consult your textbook for genetic code. Examine the following nucleotide sequence from the Me the strand of DNA what is the amino sequence of the encoded protein? ATG ccTTAC GccccT GGA GAC-GAA-AAG-AAG-GGT 2 A type of gene therapy called RNA interference (RNAi) is being investigated to treat Huntington\'s disease This disease is the result of a mutation in the DNA that results in the synthesis of a nervous system proten with an altered amino acid sequence. The mutated protein forms clumps, which causes nervous system defects. To treat this disease, scientists synthesize short sequences of RNA (siRNA, or small interferrg RNA) that forms base pairs with the mRNA that codes for the mutated protein. a Design an siRNA that will interfere with the synthesis of the protein shown in Model 2. b, Explain how the addition of the siRNA will prevent the synthesis of the mutated protein. What are the difficulties that must be overcome in order for RNA interference to be an etea technique for treating the disease?

Solution

Answer:

1).

Sense strand of DNA--------ATG-CCT-TAC-GCC-CCT-GGA-GAC-GAA-AAG-AAG-GGT

Template strand of DNA----TAC-GGA-AUG-CGG-GGA-CCT-CTG-CTT-TTC-TTC-CCA

mRNA--------------------------AUG-CCU-UAC-GCC-CCU-GGA-GAC-GAA-AAG-AAG-GGU

Peptide (amino acid chain)---Met-Pro-Tyr-Ala-Pro-Gly-Asp-Glu-Lys-Lys-Gly