The complete genome of the simplest bacterium known Mycoplas

The complete genome of the simplest bacterium known, Mycoplasma genitalium, is a circular DNA molecule with 580,070 bp. Calculate the molecular weight (assume the molecular weight of a nucleotide pair is 650) and contour length (when relaxed) of this molecule. What is Lk₀ for this Mycoplasma chromosome? If = 0.06, what is Lk?

Solution

molecular weight of the DNA = 580070*650= 377045500 dalton

Length will be same as circular DNA 580,070 bp (approx. 580 kb)

Lko= number of Base pair/10.4

=580070/10.4

=55775.96

=delta Lk/Lko

-0.06= Lk-Lko/Lko

Lk=0.94*55775.96

=52429.4024