Is this a prokaryotic or eukaryotic gene You see partial seq

Is this a prokaryotic or eukaryotic gene?

You see partial sequence of a gene as follows (non template strand shown) TATAAA(8nts)GGCCAAGGAGCCGCCATGGAACCTGAAG.. AAAAAAAAAAAAA + 1 initiation site is underlined Is this a prokaryotic or eukaryotic gene? Why? Eukaryotic gene because it has poly A tail. Prokaryotic gene because it has -10 AT rich region. Eukaryotic gene because it has a TATA box. Prokaryotic gene because it has the Shine Dalgarno sequence.

Solution

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Eukaryotic gene because it has poly A tail.

The poly-A tail is a long chain of adenine nucleotides that is added to an mRNA molecule during RNA processing to increase the stability of the molecule. Immediately after a gene in a eukaryotic cell is transcribed, the new RNA molecule undergoes several modifications known as RNA processing. The processing of the 3\' end adds a poly-A tail to the RNA molecule. First, the 3\' end of the transcript is cleaved to free a 3\' hydroxyl. Then an enzyme called poly-A polymerase adds a chain of adenine nucleotides to the RNA. This process, called polyadenylation, adds a poly-A tail that is between 100 and 250 residues long.