Suppose that you wished to determine the number of pseudogen

Suppose that you wished to determine the number of pseudogenes related to a particular gene in an organism whose complete genome had not yet been sequenced.

Part A

How might you do this experimentally? Rank the following steps in order.

1) Carry out with different primers for the RT-PCR for pseudogenes with the same restriction pattern.

2) Assume that the sequence for the gene of interest is known.

3) Clone and label DNA s.

4) Amplify part or all of the mRNA as a cDNA using reverse transcriptase-polymerase chain reaction (RT-PCR).

5) Use as a probe in Southern blotting analysis of the genome.

6) Sequence the amplified DNA s.

The correct ranking cannot be determined.