Match the type of microscopy on the left with the descriptio

Match the type of microscopy on the left with the description provided below. There is one best match _____ scans the spectrum with a focused laser beam to obtain a series of two-dimensional optical sections which be used to a focused laser beam obtain a series of an scans be specimen with dimensions. The laser excites image of the specimen in illuminated point is captured a fluorescent dye molecule, and the emitted light from each illuminated point is captured through a pinhole and recorded by a detector. confocal transmission electron phase-contrast scanning electron Match the type of microscopy on left with the corresponding description below. There is one best match. _____ the provided has the ability to resolve as small as 2 nm. confocal transmission electron fluorescence scanning electron Match the type of microscopy on the left with the corresponding description provided below. There is one best match requires coating the sample with a thin layer of a heavy metal produce three-dimensional images of the surface of sample Confocal transmission electron scanning electron phase-contrast Indicate whether the following statements are true or False. The nucleus of an animal cell is round, small, and difficult to distinguish using light microscopy True False The cytosol is fairly empty, containing a limited a number of rapid movement via diffusion. True

Solution

(6) A. Confocal

In a confocal microscope, the illumination and detection lightpaths share a common focal plane, 2 pinholes that are equidistant to the specimen.

(7)D. Scanning electron microscopy

SEMs are capable of about 2 nm resolution on biological samples. TEM can easily resolve details of 0.2nm.

(8)C.Scanning electron

SEM samples are coated with a thin layer of metal (usually gold)