You set up the following gel in an attempt to construct a re

You set up the following gel in an attempt to construct a restriction map of the plasmid pUK1, using the restriction endonucleases Ban ll and Bg/ll. This series of questions is concerned with the principles of electrophoresis underlying this study. All digests were performed on circular DNA, which if uncut give no sixe information as they are a mixture of plasmids coiled by different amounts... Assuming that all wells received the same amount of DNA, Describe the band that has the most DNA.

Solution

7. DNA separation is based on size. Small fragments travel farther than the heavier fragments.

The bands at the top of each lane represent the heavy DNA fragments (most DNA). While the bottom bands represent smallest DNA.