Match the Statements below with bacteriological tests Eosinm

Match the Statements below with bacteriological tests. Eosin-methylene blue Kovac\'s reagent reaction H_2O_2 is used Contains mannitol sugar Metabolism of tryptophan Ammonia causes color change Contains high concentration of sodium chloride Staphylococcus aureus changes it to yellow The main carbohydrate for these tests is lactose Sulfanilic acid and dimethylnaphthalamine are reagents Contains lactose, sucrose, glucose Green metallic sheen with E. coli Hydrogen sulfide is produced Acid slant, acid butt is the reaction Contains Bile salts Anaerobic organisms This medium is used to measure hemolysis Zinc is a reagent in this test Vacomycin, colisting and nystatin are inhibitors in this medium Crystan violet and lactose Detects cytochromes in bacteria Urease test TSI MSA ENB SIM Catalase test MacConkey agar Simmon citrate Oxidase test Blood agar Chocolate agar Nitrate test Thioghlycolate medium

Solution

Answer:

(i) d

Eosin methylene blue agar is abbreviated as EMB.

(ii) e

Kovac\'s reagent is used in indole test on SIM (Sulfide, Indole, Motility) medium.

(iii) f

Presence of catalase is detected using hydrogen peroxide.

(iv) c

Mannitol salt agar is a selective and differential growth media containing mannitol.

(v) e

SIM media is a differential media which contains tryptophan. If the test organism contains tryptophanase, tryptophan gets degraded.

(vi) a

Urease converts urea to ammonia to bicarbonate in urease test.

(vii) c

MSA contains 75.0 g/L sodium chloride.

(viii) c

Staphylococcus ferments mannitol and changes medium turns yellow.

(ix) g

MacConkey agar turns ping if the organism ferments lactose.

(x) l

Nitrate test is used to test for reduction of nitrate (NO₃^–) to nitrite (NO₂^–) by adding sulfanilic acid reagent and diphenylnaphthylamine.

(xi) b

TSI slant contains agar, phenol red, lactose, sucrose, glucose.

(xii) d

If E. coli grows on EMB, it will give a distinctive metallic green sheen.

(xiii) b

Hydrogen sulphide is produced in TSI slant when sugars get fermented.

(xiv) b

Acid slant, acid butt reaction is observed in TSI slant.

(xv) g

MacConkey agar contains bile salt to inhibit gram positive bacteria.

(xvi) i

Oxidase test is used to detect cytochrome system in bacteria. Anaerobic organisms lack cytochrome systems.

(xvii) j

Blood agar is used to detect hemolytic enzymes a bacteria possesses.

(xviii) l

Zinc powder is uded in nitrate test to detect the nitrate reduction enzyme in bacteria on the basis of colour.

(xix) k

Modified Thayer Martin agar is based on Chocolate II agar for the selective isolation and growth of Neisseria species.

(xx) g

MacConkey agar contains lactose and crystal violet dye to selectively isolate gram negative and enteric bacilli.

(xxi) f

Catalase is present in bacteria with cytochromes, while it lacks in bacteria where cytochrome is absent. Bacteria lacking cytochrome system cannot breakdown hydrogen peroxide due to absence of catalase.