What are the advantages of knowing the diameter of the field

What are the advantages of knowing the diameter of the field of view at a given magnification? Why must specimens viewed with a compound microscope be thin? Why are they sometimes stained with dyes? Why is depth of field important in studying biological structures? How can it affect your ability to find and examine field a specimen? What is the importance of adjusting the light intensity when viewing specimens with a compound microscope? What is the function of each of the following parts of a compound and dissecting microscope? Oculars Objectives Condenser Iris diaphragm Stage Coarse adjustment Fine adjustment Examine the micrograph of the letter e shown in figure 3.4. This letter is magnified 40x.What is the actual height of the letter?

Solution

Q .what are the advantages of knowing the diameter of the field of view at a given magnification?

Answer :

the diameter of the field of view at a given magnification, enable us to

estimate the size of an organism in the field of view in microscope.

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Q .Why must specimens viewed with a compound microscope be thin? Why are they sometimes stained with dye?

Answer :

so that it to be visible in a compound microscope.

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Why is depth of field important in studying biological structures? How can it affect your ability to find and examine a specimen?

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What is the importance of adjusting the light intensity when viewing specimens with a compound microscope?

The power of magnification increases, more intensities of light are needed to observed the speciemen.

Hence , the iris light diaphragm and condenser adjustment might be required

For

viewing quality or reducing eye strain from bright light.

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Oculars: :It is used for viewing objects on the microscope such as 10X magnification

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Objectives : It is used for the majority of the magnifying power.

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condenser lens.:

it focuses light from the light source into the objective lens.

it is located immediately below the specimen.

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Iris diaphragm:

It allows for adjustment of light intensity entering the objective lens;

If it is open, the image will be bright.

It it is closed, the image will be dim,

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Stage : it provides a flat surface for holding the slide on microscope platform.

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coarse adjustment : it enables rapid changes in focus on an image.

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fine adjustment: it enables small scale changes in focus on an image.

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